Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30628
Yamamoto H, et al., Two forms of Hox11 a T cell leukemia oncogene, are expressed in fetal spleen but not in primary lymphocytes. Mol Immunol. 1995 Nov;32(16):1177-82
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3033897
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Gene symbol:
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Tlx1
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Gene name:
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T cell leukemia, homeobox 1
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Probe:
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exon 1, exon 3
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Probe preparation:
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Double stranded labelled with digoxigenin DNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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RT-PCR product was hybridized to a Digoxigenin labelled DNA probe then visualized with an Alkaline Phosphatase labelled antibody.
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Results
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Image: 2
| E13.5
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TS21: gland
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
spleen
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| E14.5
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TS22: spleen primordium
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| E15.5
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TS23: spleen primordium
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| E16.5
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TS24: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| E17.5
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TS25: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| E18.5
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TS26: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| P1
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TS27: spleen
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Weak
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Weak
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| P w 1
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TS28: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| P w 2
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TS28: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| P w 3
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TS28: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| P w 4
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TS28: spleen
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Not Specified
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Not Specified
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| E17.5
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TS25: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
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| E17.5
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TS25: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
Sample is a 10 fold dilution of RT-PCR reaction.
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| E17.5
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TS25: spleen
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
Sample is a 50 fold dilution of RT-PCR reaction.
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| Control |
Notes:
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Analysis Tools