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Gene Expression Data
Assay Details
Assay
Reference: J:30628 Yamamoto H, et al., Two forms of Hox11 a T cell leukemia oncogene, are expressed in fetal spleen but not in primary lymphocytes. Mol Immunol. 1995 Nov;32(16):1177-82
Assay type: RT-PCR
MGI Accession ID: MGI:3033771
Gene symbol: Tlx1
Gene name: T cell leukemia, homeobox 1
Probe: exon 1, exon 3
Probe preparation: Double stranded labelled with digoxigenin DNA
Visualized with: Alkaline phosphatase
Assay notes: RT-PCR product was hybridized to a Digoxigenin labelled DNA probe then visualized with an Alkaline Phosphatase labelled antibody.
Results Image: 1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
B.A. (E10.5) E10.5 TS17: branchial arch Present Present 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified
Thymus E13.5 TS21: thymus primordium Present Present 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified
Spleen E13.5 TS21: foregut gland Present Present 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified spleen
Liver E13.5 TS21: liver Absent Absent 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified
Yolk Sac E13.5 TS21: yolk sac Absent Absent 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified
1X E17.5 TS25: spleen Present Present 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified
10X E17.5 TS25: spleen Present Present 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified Sample is a 10 fold dilution of RT-PCR reaction.
50X E17.5 TS25: spleen Present Present 1 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified Sample is a 50 fold dilution of RT-PCR reaction.
N Control
Notes:
(a) This is the shorter of 2 detected transcripts.
(b) This is the longer of 2 detected transcripts.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory