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Gene Expression Data
Assay Details
Assay
Reference: J:84436 Ono R, et al., Identification of a large novel imprinted gene cluster on mouse proximal chromosome 6. Genome Res. 2003 Jul;13(7):1696-705
Assay type: RT-PCR
MGI Accession ID: MGI:3029982
Gene symbol: Pon3
Gene name: paraoxonase 3
Probe: Pon3-pC, Pon3-pD
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: The results shown here represent amplification with 'nested' primers. The 'outer' primers used in a prior reaction are described in MGI:3029960. The final reaction product was digested with PleI. The purpose of this experiment was to determine if this gene was imprinted. Results from reciprocal mating crosses were used to determine if bias existed.
Results Image: 4D
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
B6 genome Control
JF1 genome Control
BxJ genome Control
BxJ embryo (d10) E10.0 (c) TS16: embryo Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB embryo (d10) E10.0 (c) TS16: embryo Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
BxJ placenta (d10) E10.0 (c) TS28: placenta Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB placenta (d10) E10.0 (c) TS28: placenta Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
BxJ Yolk Sac (d10) E10.0 (c) TS16: yolk sac Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB Yolk Sac (d10) E10.0 (c) TS16: yolk sac Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
BxJ embryo (d13) E13.0 (c) TS21: embryo Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB embryo (d13) E13.0 (c) TS21: embryo Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
BxJ Yolk Sac (d13) E13.0 (c) TS21: yolk sac Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB Yolk Sac (d13) E13.0 (c) TS21: yolk sac Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
BxJ liver P newborn TS27: liver Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB liver P newborn TS27: liver Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
BxJ lung P newborn TS27: lung Present Present 1 µg; total RNA (C57BL/6 x JF1)F1 Not Specified
JxB lung P newborn TS27: lung Present Present 1 µg; total RNA (JF1 x C57BL/6)F1 Not Specified
Notes:
(a) There was preferential maternal expression in E10.0 placenta and E13.0 yolk sac samples. This band represents expression from the C57BL/6 allele.
(b) There was preferential maternal expression in E10.0 placenta and E13.0 yolk sac samples. This band represents expression from the JF1 allele.
(c) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory