Gene Expression Data
Assay Details
Assay
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Reference:
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J:84436
Ono R, et al., Identification of a large novel imprinted gene cluster on mouse proximal chromosome 6. Genome Res. 2003 Jul;13(7):1696-705
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3029977
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Gene symbol:
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Casd1
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Gene name:
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CAS1 domain containing 1
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Probe:
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Cas1-pA, Cas1-pB
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Probe preparation:
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labelled with P32
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Visualized with:
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Phosphorimaging
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Assay notes:
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The RT-PCR reaction was digested with DdeI. The purpose of this experiment was to determine if this gene was imprinted. Results from reciprocal mating crosses were used to determine if bias existed.
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Results
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Image: 3B
P adult
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TS28: mouse
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Not Applicable
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Present
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1 µg; total RNA |
C57BL/6 |
| Not Specified |
P adult
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TS28: mouse
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Present
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Not Applicable
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1 µg; total RNA |
JF1 |
| Not Specified |
E10.0
(c)
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TS16: embryo
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
E10.0
(c)
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TS16: embryo
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Present
|
Present
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1 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
E10.0
(c)
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TS28: placenta
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Present
|
Present
|
1 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
E10.0
(c)
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TS28: placenta
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Present
|
Present
|
1 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
E10.0
(c)
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TS16: yolk sac
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Present
|
Present
|
1 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
E10.0
(c)
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TS16: yolk sac
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Present
|
Present
|
1 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
E13.0
(c)
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TS21: embryo
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Present
|
Present
|
1 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
E13.0
(c)
|
TS21: embryo
|
Present
|
Present
|
1 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
E13.0
(c)
|
TS21: yolk sac
|
Present
|
Present
|
1 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
E13.0
(c)
|
TS21: yolk sac
|
Present
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Present
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1 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
P newborn
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TS27: brain
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Present
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Present
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1 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
P newborn
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TS27: brain
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Present
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Present
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1 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
Notes:
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