Peg12 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:73955 Kobayashi S, et al., Paternal expression of a novel imprinted gene, Peg12/Frat3, in the mouse 7C region homologous to the Prader-Willi syndrome region. Biochem Biophys Res Commun. 2002 Jan 11;290(1):403-8
Assay type:	RT-PCR
MGI Accession ID:	MGI:2686691
Gene symbol:	Peg12
Gene name:	paternally expressed 12

Probe:	Peg12-pA, Peg12-pB
Visualized with:	Ethidium bromide
Assay notes:	The PCR product amplified contained no MboII restriction site C57BL/6 (521bp product) and one site for JF1 (338 and 177bp products). The C57BL/6 and JF1 alleles could be distinguished by digestion with MboII. This assay indicates paternal expression.

Results

Image: 2B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	521 bp (a)	521 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
C57BL/6	Control
JF1	Control
(BxJ)F1	Control
(BxJ)F1 cDNA	E10.0 (c)	TS16: embryo	Absent	Present	1 µg; total RNA	(C57BL/6 x JF1)F1		Not Specified
(BxJ)F1 cDNA	E10.0 (c)	TS16: embryo	Absent	Present	1 µg; total RNA	(C57BL/6 x JF1)F1		Not Specified
(JxB)F1	Control
(JxB)F1 cDNA	E10.0 (c)	TS16: embryo	Present	Absent	1 µg; total RNA	(JF1 x C57BL/6)F1		Not Specified
(JxB)F1 cDNA	E10.0 (c)	TS16: embryo	Present	Absent	1 µg; total RNA	(JF1 x C57BL/6)F1		Not Specified

Notes:
(a) This band represents expression from the C57BL/6 allele.
(b) This band represents expression from the JF1 allele.
(c) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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