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Gene Expression Data
Assay Details
Assay
Reference: J:87042 Krebs O, et al., Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud. Development. 2003 Dec;130(24):6037-47
Assay type: RT-PCR
MGI Accession ID: MGI:2684130
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Results
Image: 8J ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
pUC standard Control
raz/raz FL Ant E11.5 (a) TS19: forelimb bud Strong (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz FL Post E11.5 (a) TS19: forelimb bud Strong (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz HL Ant E11.5 (a) TS19: hindlimb bud Strong (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz HL Post E11.5 (a) TS19: hindlimb bud Strong (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz body E11.5 (a) TS19: trunk Present Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
wt/raz FL Ant E11.5 (a) TS19: forelimb bud Present (c) Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz FL Post E11.5 (a) TS19: forelimb bud Present (c) Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz HL Ant E11.5 (a) TS19: hindlimb bud Present (c) Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz HL Post E11.5 (a) TS19: hindlimb bud Present (c) Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz body E11.5 (a) TS19: trunk Present Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/wt FL Ant E11.5 (a) TS19: forelimb bud Weak (d) Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt FL Post E11.5 (a) TS19: forelimb bud Weak (d) Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt HL Ant E11.5 (a) TS19: hindlimb bud Weak (d) Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt HL Post E11.5 (a) TS19: hindlimb bud Weak (d) Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt body E11.5 (a) TS19: trunk Present Not Specified; total RNA involves: C3H/HeJ Not Specified
empty Lane not used in this assay
negative control Control
negative control Control
empty Lane not used in this assay
positive control Control
genomic DNA Control
empty Lane not used in this assay
Lane 24 Mol. Wt. Marker Lane
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report greatly enhanced levels of expression.
(c) Authors report enhanced levels of expression compared with wild-type.
(d) Authors state that they did not measure any huge difference in expression between anterior and posterior halves.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory