Sytl2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:69335 Fukuda M, et al., Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain. Biochem Biophys Res Commun. 2001 May 4;283(2):513-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2682590
Gene symbol:	Sytl2
Gene name:	synaptotagmin-like 2

Probe:	Slp2-a primers
Visualized with:	Ethidium bromide
Assay notes:	The authors state that the splicing events were differently regulated in different tissues, in different developmental stages, and according to isoform, and did not provide details on every band or lack thereof. This assay is the second panel from the top in figure 4A.

Results

Image: 4A Slp2-a lower

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
Heart	P adult	TS28: heart	Not Specified	Not Specified	Not Specified	Trace	Not Specified	Not Specified		Not Specified
Brain	P adult	TS28: brain	Strong	Not Specified	Not Specified	Trace	Not Specified	Not Specified		Not Specified
Spleen	P adult	TS28: spleen	Not Specified	Present	Not Specified	Trace	Not Specified	Not Specified		Not Specified
Lung	P adult	TS28: lung	Not Specified	Not Specified	Present	Trace	Not Specified	Not Specified		Not Specified
Liver	P adult	TS28: liver	Not Specified	Not Specified	Not Specified	Trace	Not Specified	Not Specified		Not Specified
Skeletal muscle	P adult	TS28: skeletal muscle	Not Specified	Not Specified	Present	Trace	Not Specified	Not Specified		Not Specified
Kidney	P adult	TS28: metanephros	Strong	Not Specified	Not Specified	Trace	Not Specified	Not Specified		Not Specified
Testis	P adult	TS28: testis	Strong	Not Specified	Not Specified	Trace	Not Specified	Not Specified		Male
E7	E7.0 (d)	TS10: embryo	Not Specified	Present	Not Specified	Trace	Not Specified	Not Specified		Not Specified
E11	E11.0 (d)	TS18: embryo	Present	Absent	Present	Trace	Not Specified	Not Specified		Not Specified
E15	E15.0 (d)	TS23: embryo	Not Specified	Not Specified	Present (e)	Trace	Not Specified	Not Specified		Not Specified
E17	E17.0 (d)	TS25: embryo	Not Specified	Not Specified	Present (e)	Trace	Not Specified	Not Specified		Not Specified
-	Control

Notes:
(a) The authors call this isoform [delta]SII.
(b) The authors call this isoform [delta]SIII.
(c) The authors call this isoform [delta]SII/[delta]SIII.
(d) Age of embryo at noon of plug day not specified in reference.
(e) There is a gradual increase in expression of this isoform from E11 to E17.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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