About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:38297 Hughes DC, et al., Cloning and sequencing of the mouse Gli2 gene: localization to the Dominant hemimelia critical region. Genomics. 1997 Jan 15;39(2):205-15
Assay type: RT-PCR
MGI Accession ID: MGI:2682274
Gene symbol: Gli2
Gene name: GLI-Kruppel family member GLI2
Probe: 12/1b
Assay notes: RT-PCR prodcuts were digested with HpaII which detects a polymorphism prior to gel electropheresis. The Dh allele of Gli2 is digested with HpaII, whereas the C57BL/6 allele is not.
Results Image: 4
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
1 Mol. Wt. Marker Lane
2 E10.5 (c) TS17: embryo Present Absent Not Specified C57BL/6 Not Specified
3 E10.5 (c) TS17: embryo Present Present Not Specified involves: C57BL/6 Dh/Dh+Not Specified
4 E10.5 (c) TS17: embryo Present Present Not Specified involves: C57BL/6 Dh/Dh+Not Specified
5 E10.5 (c) TS17: embryo Present Present Not Specified involves: C57BL/6 Dh/Dh+Not Specified
6 E10.5 (c) TS17: embryo Present Present Not Specified involves: C57BL/6 Dh/Dh+Not Specified
7 E10.5 (c) TS17: embryo Absent Present Not Specified involves: C57BL/6 Dh/DhNot Specified
8 Control
9 Mol. Wt. Marker Lane
Notes:
(a) This represents the large uncut fragment.
(b) This represents the smaller cut fragment.
(c) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory