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Gene Expression Data
Assay Details
Assay
Reference: J:46792 Greenfield A, et al., The UTX gene escapes X inactivation in mice and humans. Hum Mol Genet. 1998 Apr;7(4):737-42
Assay type: RT-PCR
MGI Accession ID: MGI:2681938
Gene symbol: Kdm6a
Gene name: lysine (K)-specific demethylase 6A
Probe: Utx-4, Utx-5
Visualized with: Ethidium bromide
Assay notes: Primers Utx-4 and Utx-5 (MGI:2681927) were used for the first 20 cycles; primers Utx-6 and Utx-7 (MGI:2681928) were then for an additional 15 cycles. Products were then digested with BstEII which recognises a site in the RT-PCR product of the M. castaneus.
Results Image: 4a Utx
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
T(X;16)16H P adult TS28: brain Present Not Applicable Not Specified Translocation stock Not Specified
M.CAST P adult TS28: brain Not Applicable Present Not Specified M. m. castaneus Not Specified
F1 female brain D Control
F1 female brain - Control
F1 female brain + P newborn TS27: brain Present Present Not Specified (Translocation stock x M. m. castaneus)F1 Female
F1 female brain D Control
F1 female brain D Control
F1 female brain + P newborn TS27: brain Present Present Not Specified (Translocation stock x M. m. castaneus)F1 Female
Notes:
(a) This band represents the RT-PCR product from the translation stock.
(b) This band represents the M. castaneus RT-PCR product; it is susceptible to digestion with BstEII.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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