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Gene Expression Data
Assay Details
Assay
Reference: J:14393 Robertson M, et al., Expression of alternative forms of differentiation inhibiting activity (DIA/LIF) during murine embryogenesis and in neonatal and adult tissues. Dev Genet. 1993;14(3):165-73
Assay type: RNase protection
MGI Accession ID: MGI:2681096
Gene symbol: Lif
Gene name: leukemia inhibitory factor
Probe: DIA/LIF probe
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Author states that strains C57BL/6, 129, MF1 and PO albino outbreds were used with no differences in expression observed between strains.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
0.1 Control
1.0 Control
P Control
tRNA Control
Int P adult TS28: intestine Present (b) 15 µg; total RNA Not Specified Not Specified
Lung P adult TS28: lung Present 15 µg; total RNA Not Specified Not Specified
Thy P adult TS28: thymus Not Specified 15 µg; total RNA Not Specified Not Specified
Br P adult TS28: brain Present (c) 15 µg; total RNA Not Specified Not Specified
Liv P adult TS28: liver Absent 15 µg; total RNA Not Specified Not Specified
Kid P adult TS28: metanephros Trace 15 µg; total RNA Not Specified Not Specified
Spl P adult TS28: spleen Not Specified 15 µg; total RNA Not Specified Not Specified
Ov P adult TS28: ovary Strong 15 µg; total RNA Not Specified Female
Ovid P adult TS28: oviduct Strong (d) 15 µg; total RNA Not Specified Female
Ut P adult TS28: uterus Strong 15 µg; total RNA Not Specified Female non-estrous virgin mouse
Tes P adult TS28: epididymis
TS28: testis 		Not Specified 15 µg; total RNA Not Specified Male
Fe P adult TS28: femur Present (e) 15 µg; total RNA Not Specified Not Specified
Sem P adult TS28: seminal vesicle Trace 15 µg; total RNA Not Specified Male
Sem P adult TS28: seminal vesicle Trace 15 µg; total RNA Not Specified Male
Smax P adult TS28: submandibular gland Trace 15 µg; total RNA Not Specified Not Specified
Eye P adult TS28: eye Present (f) 15 µg; total RNA Not Specified Not Specified
Notes:
(a) Authors state that there are 2 transcripts, D and M of 368 and 345bp, respectively.
(b) The M transcript signal is higher than the D transcript in intestine.
(c) Only the M transcript is present in brain.
(d) The M transcript signal is higher than the D transcript in oviduct.
(e) The D transcript signal is higher than the M transcript in femur.
(f) The M transcript signal is higher than the D transcript in eye.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory