Pecam1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:51965 Sheibani N, et al., Tissue specific expression of alternatively spliced murine PECAM-1 isoforms. Dev Dyn. 1999 Jan;214(1):44-54
Assay type:	RT-PCR
MGI Accession ID:	MGI:2680813
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Probe:	Pecam-1-pK, Pecam-1-pL
Visualized with:	Ethidium bromide
Assay notes:	The RT-PCR products were cloned and sequenced to determine the distribution of isoforms in tissues; results were detailed in Table 1.

Results

Image: 2 ©

	Sample Information		Bands								Other Sample Information
Lane	Age	Structure	350 bp (a)	327 bp (b)	296 bp (c)	293 bp (d)	273 bp (e)	270 bp (f)	239 bp (g)	216 bp (h)	Amount	Genetic Background	Mutant Allele(s)	Sex
Control	Control
Kidney 28d	P28	TS28: metanephros	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	0.5 µg; total RNA	CD-1		Not Specified
Kidney 9d	P9	TS28: metanephros	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	Not Specified	0.5 µg; total RNA	CD-1		Not Specified
Lung	P w 3	TS28: lung	Present	Present	Absent	Present	Present	Present	Absent	Present	0.5 µg; total RNA	CD-1		Not Specified
Kidney	P w 3	TS28: metanephros	Present	Present	Absent	Present	Present	Present	Absent	Present	0.5 µg; total RNA	CD-1		Not Specified
M	Mol. Wt. Marker Lane
Liver	P w 3	TS28: liver	Present	Present	Absent	Present	Absent	Present	Absent	Absent	0.5 µg; total RNA	CD-1		Not Specified
Heart	P w 3	TS28: heart	Present	Present	Present	Present	Present	Present	Absent	Present	0.5 µg; total RNA	CD-1		Not Specified
Brain 2	P w 3	TS28: brain	Present	Present	Absent	Present	Present	Present	Present	Present	0.5 µg; total RNA	CD-1		Not Specified
Brain 1	P w 3	TS28: brain	Present	Present	Absent	Present	Present	Present	Present	Present	0.5 µg; total RNA	CD-1		Not Specified

Notes:
(a) This band represents the full length isoform.
(b) This band represents the isoform lacking exon 15.
(c) This band represents the isoform lacking exon 12.
(d) This band represents the isoform lacking exon 14.
(e) This band represents the isoform lacking exons 12 and 15.
(f) This band represents the isoform lacking exons 14 and 15.
(g) This band represents the isoform lacking exons 12 and 14.
(h) This band represents the isoform lacking exons 12, 14 and 15.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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