Gjc1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70957 Sohl G, et al., The mouse gap junction gene connexin29 is highly expressed in sciatic nerve and regulated during brain development. Biol Chem. 2001 Jun;382(6):973-8
Assay type:	Northern blot
MGI Accession ID:	MGI:2679010
Gene symbol:	Gjc1
Gene name:	gap junction protein, gamma 1

Probe:	Cx45 probe
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The densitometric analysis of this blot is presented in Table 2.

Results

Image: 4c Cx45

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.2 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
14 dpc	E14.0 (a)	TS22: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
16 dpc	E16.0 (a)	TS24: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
18 dpc	E18.0 (a)	TS26: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
P0	P0	TS27: brain	Present (c)	20 µg; total RNA	CD-1		Not Specified
P3	P3	TS27: brain	Present (d)	20 µg; total RNA	CD-1		Not Specified
P7	P7	TS28: brain	Present (e)	20 µg; total RNA	CD-1		Not Specified
P14	P14	TS28: brain	Present (f)	20 µg; total RNA	CD-1		Not Specified
Brain adult	P adult	TS28: brain	Present (g)	20 µg; total RNA	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This was one of the times of peak expression.
(c) Expression levels are 52% of the maximum level observed.
(d) Expression levels are 67% of the maximum level observed.
(e) Expression levels are 48% of the maximum level observed.
(f) Expression levels are 6% of the maximum level observed.
(g) Expression levels are 5% of the maximum level observed.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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