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Gene Expression Data
Assay Details
Assay
Reference: J:58384 Terasawa Y, et al., Apolipoprotein B-related gene expression and ultrastructural characteristics of lipoprotein secretion in mouse yolk sac during embryonic development. J Lipid Res. 1999 Nov;40(11):1967-77
Assay type: RNase protection
MGI Accession ID: MGI:2671392
Gene symbol: Apob
Gene name: apolipoprotein B
Probe: ApoB cDNA4
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Results
Image: 1_Apo-B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 245 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 Control
Lane 2 Control
Lane 3 E7.5 (b) TS11: conceptus Present 10 µg; total RNA C57BL/6J Pooled
Lane 4 E13.5 (b) TS21: yolk sac Present 10 µg; total RNA C57BL/6J Not Specified
Lane 5 E13.5 (b) TS28: placenta Not Specified 10 µg; total RNA C57BL/6J Female
Lane 6 E13.5 (b) TS21: liver Weak 10 µg; total RNA C57BL/6J Not Specified
Lane 7 E18.5 (b) TS26: yolk sac Present 10 µg; total RNA C57BL/6J Not Specified
Lane 8 E18.5 (b) TS26: yolk sac Present (c) 10 µg; total RNA C57BL/6J Not Specified yolk sac near the placenta.
Lane 9 E18.5 (b) TS28: placenta Trace 10 µg; total RNA C57BL/6J Female
Lane 10 E18.5 (b) TS26: liver Weak 10 µg; total RNA C57BL/6J Not Specified
Lane 11 P adult TS28: liver Present 10 µg; total RNA C57BL/6J Not Specified
Lane 12 P adult TS28: metanephros Present 10 µg; total RNA C57BL/6J Not Specified
Lane 13 P adult TS28: musculature Not Specified 10 µg; total RNA C57BL/6J Not Specified
Lane 14 P adult TS28: lung Not Specified 10 µg; total RNA C57BL/6J Not Specified
Notes:
(a) Expression levels in yolk sac were lower than in adult liver after the blot was standardized for RNA loading.
(b) Age of embryo at noon of plug day not specified in reference.
(c) Expression in yolk sac near placenta was higher than in more distant samples.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory