Rag1 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:39086 Marcos MA, et al., Antigenic phenotype and gene expression pattern of lymphohemopoietic progenitors during early mouse ontogeny. J Immunol. 1997 Mar 15;158(6):2627-37
Assay type:	RT-PCR
MGI Accession ID:	MGI:2670408
Gene symbol:	Rag1
Gene name:	recombination activating 1

Probe:	F3, R6
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The RT-PCR reaction was electrophoresed, Southern blotted and hybridized with a probe (MGI:2670373). Additionally, the data from this blot was plotted on a bar graph in Figure 5E as the relative message level compared to the control for the ages assayed.

Results

Image: 5D RAG-1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
10.5	E11.0 (a)	TS18: liver	Not Specified	0.1-1 µg; total RNA	BALB/c		Not Specified
11	E11.5 (a)	TS19: liver	Not Specified	0.1-1 µg; total RNA	BALB/c		Not Specified
12	E12.5 (a)	TS20: liver	Present	0.1-1 µg; total RNA	BALB/c		Not Specified
13	E13.5 (a)	TS21: liver	Present	0.1-1 µg; total RNA	BALB/c		Not Specified
14	E14.5 (a)	TS22: liver	Present	0.1-1 µg; total RNA	BALB/c		Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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