Antibody:	anti-TAK1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: embryo	Weak	Uniform

Structure	Level	Pattern	Image	Note
TS18: embryo	Weak	Uniform	1A
TS18: dorsal root ganglion	Strong		1A
TS18: future spinal cord	Strong	Regionally restricted	1A	Expression is in the ventral regions extending the entire length of the cord.
TS18: pancreas primordium	Strong	Regionally restricted	1A	Expression is in the epithelial buds.
TS18: hindbrain	Strong	Regionally restricted	1A	Expression is in the roof of the hindbrain and in areas of differentiating cells surrounding the 4th ventricle.
TS18: forebrain	Strong	Regionally restricted	1A	Expression is in the non-proliferative areas of the neuroepithelium.
TS18: telencephalon	Strong	Regionally restricted	1A	Expression in the outer-most layer.
TS18: diencephalon	Strong		1A
TS18: gut	Strong	Regionally restricted	1A	Expression is in the epithelial layers.
TS18: main bronchus epithelium	Strong		1A

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		1B
TS21: spinal cord	Present	Regionally restricted	1B	Expression is in the ventral regions extending the entire length of the cord.
TS21: hindbrain	Present	Regionally restricted	1B	Expression is in areas of differentiating cells surrounding the 4th ventricle and absent in the ventricular zone.
TS21: choroid plexus	Present		1B
TS21: diencephalon	Present		1B
TS21: telencephalon	Present		1B
TS21: nose	Present	Regionally restricted	1B	Expression is in the nasal epithelium.
TS21: gut	Present	Regionally restricted	1B	Expression is in the epithelial layer.
TS21: pancreas	Strong		1B

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	1C	Expression is in areas of differentiating cells surrounding the 4th ventricle and absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: mesonephros	Present	Regionally restricted		Expression is in the epithelial tubules, ureteric bud.
TS21: metanephros	Present	Regionally restricted		Expression is in the epithelial tubules, ureteric bud.
TS21: liver	Present	Patchy

Structure	Cell Type	Level	Pattern	Image	Note
TS22: metanephros		Present	Regionally restricted		Expression in all epithelial tissues as well as in areas of condensing metanephric mesenchyme adjacent to the branching tips of the ureteric bud.
TS22: germ cell of testis		Present	Regionally restricted		Expression in the testicular cords is in both germ cells and Sertoli cells.
TS22: Sertoli cell	Sertoli cell	Present	Regionally restricted		Expression in the testicular cords is in both germ cells and Sertoli cells.

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present		2B

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	2C	Expression is in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	2D	Expression is in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Strong		2E

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Strong		2F

Structure	Level	Pattern	Image	Note
TS23: metanephros	Strong	Regionally restricted	3A	Expression is in the epithelia and condensing mesenchyme.
TS23: maturing glomerular tuft	Weak		3A

Structure	Level	Pattern	Image	Note
TS23: metanephros	Strong	Regionally restricted	3B	Expression is in the epithelia and condensing mesenchyme.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: germ cell of testis		Strong		3C	Expression is in both germ cells and Sertoli cells.
TS23: Sertoli cell	Sertoli cell	Strong		3C	Expression is in both germ cells and Sertoli cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: germ cell of testis		Strong		3D	Expression is in both germ cells and Sertoli cells.
TS23: Sertoli cell	Sertoli cell	Strong		3D	Expression is in both germ cells and Sertoli cells.

Structure	Level	Pattern	Image	Note
TS23: duodenum caudal part epithelium	Strong		3E
TS23: foregut-midgut junction epithelium	Strong		3E

Structure	Level	Pattern	Image	Note
TS23: duodenum caudal part epithelium	Present		3F
TS23: foregut-midgut junction epithelium	Present		3F

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		3G

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Patchy	3H	Epithelial cells show the highest level of expression and it is somewhat lower in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: liver	Present	Patchy	3I

Structure	Level	Pattern	Image	Note
TS23: stomach epithelium	Strong
TS23: gut	Strong	Regionally restricted		Expression in the epithelial lining.