Ewsr1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70044 Melot T, et al., Characterization of a new brain-specific isoform of the EWS oncoprotein. Eur J Biochem. 2001 Jun;268(12):3483-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2667661
Gene symbol:	Ewsr1
Gene name:	Ewing sarcoma breakpoint region 1

Probe:	ISON1, ISON2
Probe preparation:	DNA
Assay notes:	Competitive PCR by co-amplification of isoN and GAPDH transcripts.

Results

Image: 4C

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E11.5	E11.5 (a)	TS19: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
E12.5	E12.5 (a)	TS20: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
E13.5	E13.5 (a)	TS21: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
E14.5	E14.5 (a)	TS22: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
E15.5	E15.5 (a)	TS23: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
E16.5	E16.5 (a)	TS24: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
E17.5	E17.5 (a)	TS25: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
PN	P newborn	TS27: brain	Present (c)	1 µg; total RNA	Not Specified		Not Specified
adult	P adult	TS28: brain	Present (d)	1 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Labeled isoN.
(c) Authors report that the expression level of isoN was stable from E11.5 to birth.
(d) Authors state that expression was slightly lower in adult than in newborn brains.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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