Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Rasgrf1tm1Esn
Name:	RAS protein-specific guanine nucleotide-releasing factor 1; targeted mutation1, Eugenio Santos
MGI ID:	MGI:2667231
Synonyms:	GRF1-
Gene:	Rasgrf1  Location: Chr9:89791961-89909030 bp, + strand  Genetic Position: Chr9, 47.31 cM
Alliance:	Rasgrf1tm1Esn page

Germline Transmission:	Earliest citation of germline transmission: J:83950
Parent Cell Line:	RW-4 (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Exons 5 and 6 in the catalytic domain were replaced with a PGKneomycin cassette. This inactivates all potential splicing isoforms. Proper insertion and absence of expression were demonstrated by Sothern blot analysis and RT-PCR. Absence of protein was confirmed by immunoblotting. (J:83950)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rasgrf1 Mutation:	65 strains or lines available

Original:	J:83950 Font de Mora J, et al., Ras-GRF1 signaling is required for normal beta-cell development and glucose homeostasis. EMBO J. 2003 Jun 16;22(12):3039-49
All:	8 reference(s)