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Gene Expression Data
Assay Details
Assay
Reference: J:54776 Hiroi H, et al., Stage-specific expression of estrogen receptor subtypes and estrogen responsive finger protein in preimplantational mouse embryos. Endocr J. 1999 Feb;46(1):153-8
Assay type: RT-PCR
MGI Accession ID: MGI:2663983
Gene symbol: Trim25
Gene name: tripartite motif-containing 25
Probe: efp-pC, efp-pD
Probe preparation: DNA
Assay notes: Nested PCR with primer sets efp-pA and -pB (MGI:2663976), and efp-pC and -pD (MGI:2663977).
Results Image: 3
 Sample Information Bands Other Sample Information
Lane AgeStructure 585 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
M Mol. Wt. Marker Lane
ovary P adult TS28: ovary Present 0.5 µg; total RNA ICR Female
cumulus+oocyte P adult TS28: cumulus oophorus
TS28: oocyte 		Present 0.5 µg; total RNA ICR Female Cumulus-oocyte complexes, not treated with hyaluronidase.
denuded oocyte P adult TS28: oocyte Absent 0.5 µg; total RNA ICR Female Treated with hyaluronidase to remove the cumulus.
2-cell E1.0 (a) TS2: 2-cell stage conceptus Absent 0.5 µg; total RNA ICR Not Specified
4-cell E2.0 (a) TS3: 4-cell stage conceptus Present 0.5 µg; total RNA ICR Not Specified
8-cell E2.5 (a) TS3: 8-cell stage conceptus Absent 0.5 µg; total RNA ICR Not Specified
morulae E3.0 (a) TS3: morula-stage conceptus Present 0.5 µg; total RNA ICR Not Specified
blastocyst E3.5 (a) TS4: blastocyst-stage conceptus Present 0.5 µg; total RNA ICR Not Specified
Notes:
(a) Embryonic age assigned by curator based on morphologic criteria.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory