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Gene Expression Data
Assay Details
Assay
Reference: J:53778 Fantin VR, et al., Cloning, tissue expression, and chromosomal location of the mouse insulin receptor substrate 4 gene. Endocrinology. 1999 Mar;140(3):1329-37
Assay type: Western blot
MGI Accession ID: MGI:2663907
Gene symbol: Irs4
Gene name: insulin receptor substrate 4
Antibody: IRS-4 antibody
Detection system: Secondary antibody
Assay notes: Tissue extracts were first immunoprecipitated, then immunoblotted with the same antibody. Authors also report that no Irs4 protein was detected in any tissue after direct immunoblotting.
Results Image: 5
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
1 Control
2 Control
3 Control
4 Control
WAT W P adult TS28: white fat Absent Not Specified Not Specified
WAT K P adult TS28: white fat Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
BAT W P adult TS28: brown fat Absent Not Specified Not Specified
BAT K P adult TS28: brown fat Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
SKM W P adult TS28: skeletal muscle Absent Not Specified Not Specified
SKM K P adult TS28: skeletal muscle Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
HRT W P adult TS28: heart Absent Not Specified Not Specified
HRT K P adult TS28: metanephros Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
LUN W P adult TS28: lung Absent (a) Not Specified Not Specified
LUN K P adult TS28: lung Absent (a) involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
PAN W P adult TS28: pancreas Absent (a) Not Specified Not Specified
PAN K P adult TS28: pancreas Absent (a) involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
5 Control
6 Control
7 Control
8 Control
LIV W P adult TS28: liver Absent Not Specified Not Specified
LIV K P adult TS28: liver Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
KID W P adult TS28: metanephros Absent Not Specified Not Specified
KID K P adult TS28: metanephros Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
BRN W P adult TS28: brain Absent (a) Not Specified Not Specified
BRN K P adult TS28: brain Absent (a) involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
SPL W P adult TS28: spleen Absent Not Specified Not Specified
SPL K P adult TS28: spleen Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
THY W P adult TS28: thymus Absent Not Specified Not Specified
THY K P adult TS28: thymus Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/Irs4tm1LhdNot Specified
TES W P adult TS28: testis Absent Not Specified Male
TES K P adult TS28: testis Absent involves: 129S4/SvJae * BALB/c Irs4tm1Lhd/YMale
Notes:
(a) Authors report that a few tissues (lung, brain and pancreas) showed a weak band of approximately the expected size, but a band of equal intensity was also present in samples from knockout mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory