Irs4 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:53778 Fantin VR, et al., Cloning, tissue expression, and chromosomal location of the mouse insulin receptor substrate 4 gene. Endocrinology. 1999 Mar;140(3):1329-37
Assay type:	RT-PCR
MGI Accession ID:	MGI:2663902
Gene symbol:	Irs4
Gene name:	insulin receptor substrate 4

Probe:	Irs4-pA, Irs4-pB
Probe preparation:	DNA
Visualized with:	Ethidium bromide
Assay notes:	1 + and 2 + denote first and second amplifications with reverse transcriptase.

Results

Image: 4

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	176 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
(no label)	Mol. Wt. Marker Lane
Day 7, 1 +	E7.0 (a)	TS10: embryo	Absent	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 7, 2 +	E7.0 (a)	TS10: embryo	Absent	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 7, 1 -	Control
Day 7, 2 -	Control
Day 11, 1 +	E11.0 (a)	TS18: embryo	Absent	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 11, 2 +	E11.0 (a)	TS18: embryo	Absent	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 11, 1 -	Control
Day 11, 2 -	Control
(no label)	Mol. Wt. Marker Lane
Day 15, 1 +	E15.0 (a)	TS23: embryo	Present	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 15, 2 +	E15.0 (a)	TS23: embryo	Present	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 15, 1 -	Control
Day 15, 2 -	Control
Day 17, 1 +	E17.0 (a)	TS25: embryo	Present	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 17, 2 +	E17.0 (a)	TS25: embryo	Present	0.5 µg; poly-A+ RNA	Not Specified		Not Specified
Day 17, 1 -	Control
Day 17, 2 -	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.

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