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Gene Expression Data
Assay Details
Assay
Reference: J:53778 Fantin VR, et al., Cloning, tissue expression, and chromosomal location of the mouse insulin receptor substrate 4 gene. Endocrinology. 1999 Mar;140(3):1329-37
Assay type: RT-PCR
MGI Accession ID: MGI:2663901
Gene symbol: Irs4
Gene name: insulin receptor substrate 4
Probe: Irs4-pA, Irs4-pB
Probe preparation: DNA
Visualized with: Ethidium bromide
Assay notes: 1 + and 2 + denote first and second amplifications with reverse transcriptase.
Results Image: 3
 Sample Information Bands Other Sample Information
Lane AgeStructure 176 bp Amount Genetic BackgroundMutant Allele(s)Sex
(no label) Mol. Wt. Marker Lane
Sk. Muscle 1 + P adult TS28: skeletal muscle Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Sk. Muscle 2 + P adult TS28: skeletal muscle Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Sk. Muscle 1 - Control
Sk. Muscle 2 - Control
Brain 1 + P adult TS28: brain Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Brain 2 + P adult TS28: brain Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Brain 1 - Control
Brain 2 - Control
Pancreas 1 + P adult TS28: pancreas Absent 0.5 µg; poly-A+ RNA Not Specified Not Specified
Pancreas 2 + P adult TS28: pancreas Absent 0.5 µg; poly-A+ RNA Not Specified Not Specified
Pancreas 1 - Control
Pancreas 2 - Control
(no label) Mol. Wt. Marker Lane
Heart 1+ P adult TS28: heart Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Heart 2 + P adult TS28: heart Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Heart 1 - Control
Heart 2 - Control
Kidney 1 + P adult TS28: metanephros Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Kidney 2 + P adult TS28: metanephros Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Kidney 1 - Control
Kidney 2 - Control
Liver 1 + P adult TS28: liver Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Liver 2 + P adult TS28: liver Present 0.5 µg; poly-A+ RNA Not Specified Not Specified
Liver 1 - Control
Liver 2 - Control
(no label) Mol. Wt. Marker Lane
Lung 1 + P adult TS28: lung Absent 0.5 µg; poly-A+ RNA Not Specified Not Specified
Lung 2 + P adult TS28: lung Absent 0.5 µg; poly-A+ RNA Not Specified Not Specified
Lung 1 - Control
Lung 2 - Control
Testis 1 + P adult TS28: testis Absent 0.5 µg; poly-A+ RNA Not Specified Male
Testis 2 + P adult TS28: testis Trace (a) 0.5 µg; poly-A+ RNA Not Specified Male
Testis 1 - Control
Testis Control
Spleen 1 + P adult TS28: spleen Absent 0.5 µg; poly-A+ RNA Not Specified Not Specified
Spleen 2 + P adult TS28: spleen Absent 0.5 µg; poly-A+ RNA Not Specified Not Specified
Spleen 1 - Control
Spleen 2 - Control
Notes:
(a) Authors state that the amount was somewhat greater in reaction containing reverse transcriptase than in control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory