Irs3 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:43854 Sciacchitano S, et al., Cloning, tissue expression, and chromosomal localization of the mouse IRS-3 gene. Endocrinology. 1997 Nov;138(11):4931-40
Assay type:	Northern blot
MGI Accession ID:	MGI:2663667
Gene symbol:	Irs3
Gene name:	insulin receptor substrate 3

Probe:	Irs3 721-1178
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging

Results

Image: 5A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.4 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
7 DAYS	E7.0 (a)	TS10: embryo	Present (b)	2 µg; poly-A+ RNA	Not Specified		Not Specified
11 DAYS	E11.0 (a)	TS18: embryo	Present (c)	2 µg; poly-A+ RNA	Not Specified		Not Specified
15 DAYS	E15.0 (a)	TS23: embryo	Present (c)	2 µg; poly-A+ RNA	Not Specified		Not Specified
17 DAYS	E17.0 (a)	TS25: embryo	Present (c)	2 µg; poly-A+ RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report maximal expression at 7 days.
(c) Authors state there is a progressive reduction in levels of expression later in embryonic life.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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