Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:40036
Niedermeyer J, et al., Mouse fibroblast activation protein: Molecular cloning, alternative splicing and expression in the reactive stroma of epithelial cancers. Int J Cancer. 1997 MAY 2;71(3):383-389
|
Assay type:
|
RT-PCR
|
MGI Accession ID:
|
MGI:2662776
|
Gene symbol:
|
Fap
|
Gene name:
|
fibroblast activation protein
|
Probe:
|
hfap-15, mfap-3
|
Probe preparation:
|
labelled with P32 DNA
|
Assay notes:
|
The RT-PCR reaction was electrophoresed, Southern blotted and probed with an exon 3 specific oligo probe (see MGI:2662811). The probe used in this assay was 5' labelled.
|
|
Results
|
Image: 3B Exon 3 probe
E7.0
|
TS10: embryo
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E11.0
|
TS18: embryo
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E11.0
|
TS28: placenta
|
Not Specified
|
2.5 µg; total RNA |
NMRI |
| Female |
|
P
|
TS28: uterus
|
Not Specified
|
2.5 µg; total RNA |
NMRI |
| Female |
pregnant mouse at E11
|
E11.0
|
TS18: embryo
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E14.0
|
TS22: embryo
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E14.0
|
TS22: head
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E14.0
|
TS22: trunk
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E14.0
|
TS28: placenta
|
Not Specified
|
2.5 µg; total RNA |
NMRI |
| Female |
|
P
|
TS28: uterus
|
Not Specified
|
2.5 µg; total RNA |
NMRI |
| Female |
pregnant mouse at E14
|
E17.0
|
TS25: head
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E17.0
|
TS25: trunk
|
Present
|
2.5 µg; total RNA |
NMRI |
| Not Specified |
|
E17.0
|
TS28: placenta
|
Not Specified
|
2.5 µg; total RNA |
NMRI |
| Female |
|
P
|
TS28: uterus
|
Not Specified
|
2.5 µg; total RNA |
NMRI |
| Female |
pregnant mouse at E17
|
Notes:
|