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Gene Expression Data
Assay Details
Assay
Reference: J:40036 Niedermeyer J, et al., Mouse fibroblast activation protein: Molecular cloning, alternative splicing and expression in the reactive stroma of epithelial cancers. Int J Cancer. 1997 MAY 2;71(3):383-389
Assay type: RT-PCR
MGI Accession ID: MGI:2662776
Gene symbol: Fap
Gene name: fibroblast activation protein
Probe: hfap-15, mfap-3
Probe preparation: labelled with P32 DNA
Assay notes: The RT-PCR reaction was electrophoresed, Southern blotted and probed with an exon 3 specific oligo probe (see MGI:2662811). The probe used in this assay was 5' labelled.
Results Image: 3B Exon 3 probe
 Sample Information Bands Other Sample Information
Lane AgeStructure 409 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 E7.0 TS10: embryo Present 2.5 µg; total RNA NMRI Not Specified
Lane 2 E11.0 TS18: embryo Present 2.5 µg; total RNA NMRI Not Specified
Lane 3 E11.0 TS28: placenta Not Specified 2.5 µg; total RNA NMRI Female
Lane 4 P TS28: uterus Not Specified 2.5 µg; total RNA NMRI Female pregnant mouse at E11
Lane 5 E11.0 TS18: embryo Present 2.5 µg; total RNA NMRI Not Specified
Lane 6 E14.0 TS22: embryo Present 2.5 µg; total RNA NMRI Not Specified
Lane 7 E14.0 TS22: head Present 2.5 µg; total RNA NMRI Not Specified
Lane 8 E14.0 TS22: trunk Present 2.5 µg; total RNA NMRI Not Specified
Lane 9 E14.0 TS28: placenta Not Specified 2.5 µg; total RNA NMRI Female
Lane 10 P TS28: uterus Not Specified 2.5 µg; total RNA NMRI Female pregnant mouse at E14
Lane 11 E17.0 TS25: head Present 2.5 µg; total RNA NMRI Not Specified
Lane 12 E17.0 TS25: trunk Present 2.5 µg; total RNA NMRI Not Specified
Lane 13 E17.0 TS28: placenta Not Specified 2.5 µg; total RNA NMRI Female
Lane 14 P TS28: uterus Not Specified 2.5 µg; total RNA NMRI Female pregnant mouse at E17
Notes:
(a) Authors state that expression was detected in most embryonic tissues but was weak or absent in adult tissues.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory