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Gene Expression Data
Assay Details
Assay
Reference: J:48216 Gunes C, et al., Embryonic lethality and liver degeneration in mice lacking the metal-responsive transcriptional activator MTF-1. EMBO J. 1998 May 15;17(10):2846-54
Assay type: RT-PCR
MGI Accession ID: MGI:2656958
Gene symbol: Gclc
Gene name: glutamate-cysteine ligase, catalytic subunit
Probe: gamma GCS-pA, gamma GCS-pB
Probe preparation: DNA
Visualized with: Ethidium bromide
Results Image: 4D_gammaGCS
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
M Mol. Wt. Marker Lane
25 ng E13.5 (a) TS21: embryo Present 0.025 µg involves: 129 * C57BL/6 Not Specified
25 ng +/- E13.5 (a) TS21: embryo Present 0.025 µg involves: 129 * C57BL/6 Mtf1tm1Wsc/Mtf1+Not Specified
25 ng -/- E13.5 (a) TS21: embryo Present (b) 0.025 µg involves: 129 * C57BL/6 Mtf1tm1Wsc/Mtf1tm1WscNot Specified
M Mol. Wt. Marker Lane
100 ng +/+ E13.5 (a) TS21: embryo Present 0.1 µg involves: 129 * C57BL/6 Not Specified
100 ng +/- E13.5 (a) TS21: embryo Present 0.1 µg involves: 129 * C57BL/6 Mtf1tm1Wsc/Mtf1+Not Specified
100 ng -/- E13.5 (a) TS21: embryo Present (b) 0.1 µg involves: 129 * C57BL/6 Mtf1tm1Wsc/Mtf1tm1WscNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report reduced expression compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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