Pou2f1 RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:3740 Suzuki N, et al., Mouse Oct-1 contains a composite homeodomain of human Oct-1 and Oct-2. Nucleic Acids Res. 1993 Jan 25;21(2):245-52
Assay type:	RNase protection
MGI Accession ID:	MGI:2656902
Gene symbol:	Pou2f1
Gene name:	POU domain, class 2, transcription factor 1

Probe:	590 nt Probe
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Results

Image: 3B

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	590 bp (a)	389 bp (b)	129 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
marker	Mol. Wt. Marker Lane
probe (590 nt)	Control
F9 (UD)	In vitro data: no data stored
F9 (D)	In vitro data: no data stored
emb (day 13)	E13.0 (c)	TS21: embryo	Present	Present	Present	30 µg; total RNA	Not Specified		Not Specified
brain	P adult	TS28: brain	Present	Present	Present	30 µg; total RNA	Not Specified		Not Specified
thymus	P adult	TS28: thymus	Present	Present	Present	30 µg; total RNA	Not Specified		Not Specified
heart	P adult	TS28: heart	Present	Present	Present	30 µg; total RNA	Not Specified		Not Specified
liver	P adult	TS28: liver	Present	Present	Present	30 µg; total RNA	Not Specified		Not Specified
intestine	P adult	TS28: intestine	Present	Present	Present	30 µg; total RNA	Not Specified		Not Specified
testis	P adult	TS28: testis	Present	Present	Present	30 µg; total RNA	Not Specified		Male

Notes:
(a) This band is diagnostic for the Oct1A transcript.
(b) These bands are diagnostic for the Oct1B and Oct1C transcripts; they are predominant.
(c) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23