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Gene Expression Data
Assay Details
Assay
Reference: J:22646 Tsumaki N, et al., Differential expression of an acidic domain in the amino-terminal propeptide of mouse pro-alpha 2(XI) collagen by complex alternative splicing. J Biol Chem. 1995 Feb 3;270(5):2372-8
Assay type: RT-PCR
MGI Accession ID: MGI:2656799
Gene symbol: Col11a2
Gene name: collagen, type XI, alpha 2
Probe: Primer A, Primer B
Assay notes: The RT-PCR reaction, which amplified the cDNA region corresponding to exons 5-10 was electrophoresed (see Figure 5B) and Southern blotted with an ECL 3'-oligolabeling and detection system. The probe was from exon 8 sequence (MGI:2656770).
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 489 bp (a) 426 bp (b) 348 bp (c) Amount Genetic BackgroundMutant Allele(s)Sex
Size Marker Mol. Wt. Marker Lane
Cartilage P w 3 TS28: cartilage Absent Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Muscle P w 3 TS28: skeletal muscle Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Brain P w 3 TS28: brain Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Heart P w 3 TS28: heart Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Eye P w 3 TS28: eye Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Calvaria P w 3 TS28: skeletal system Not Specified Not Specified Present 5 µg; total RNA Not Specified Not Specified
Liver P w 3 TS28: liver Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Size Marker Mol. Wt. Marker Lane
10-d-p.c. E10.0 (d) TS16: forelimb bud Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
11-d-p.c. E11.0 (d) TS18: forelimb bud Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
12-d-p.c. E12.0 (d) TS20: forelimb Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
14-d-p.c. E14.0 (d) TS22: forelimb Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
3-w-o cartilage P w 3 TS28: cartilage Absent Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
Size Marker Mol. Wt. Marker Lane
Notes:
(a) This splice variant contains exons 5,6,7, 8 and 9 and is one of the major combinations of alternatively spliced exons. This band was expressed in most tissues except cartilage.
(b) This may have been 426 or 411bp. The splice variant contained exons 5, (6 or 7), 8 and 9 and may represent a low level of aberrant splicing.
(c) This splice variant contained exons 5, 8 and 9.
(d) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory