Hoxa1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:33943 Baron A, et al., Hox-1.6: a mouse homeo-box-containing gene member of the Hox-1 complex. EMBO J. 1987 Oct;6(10):2977-86
Assay type:	Northern blot
MGI Accession ID:	MGI:2656089
Gene symbol:	Hoxa1
Gene name:	homeobox A1

Probe:	Hox-1.6 cDNA 3
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	Authors report that the same result was obtained when similar Northern blots were hybridized with an antisense RNA probe.

Results

Image: 4B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	10 kb	4.5 kb (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
D9	E9.0 (a)	TS14: embryo	Present (b)	Present	30 µg; total RNA	Not Specified		Not Specified
D10	E10.0 (a)	TS16: embryo	Present	Present	30 µg; total RNA	Not Specified		Not Specified
D13	E13.0 (a)	TS21: embryo	Absent (d)	Absent	30 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Strongest signal at day 9.
(c) Authors state that the 4.5 kb bands disappeared under very stringent conditions and may not correspond to a genuine transcript.
(d) Undetectable by day 13.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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