Il11ra1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:33356 Robb L, et al., Structural analysis of the gene encoding the murine interleukin-11 receptor alpha-chain and a related locus. J Biol Chem. 1996 Jun 7;271(23):13754-61
Assay type:	RT-PCR
MGI Accession ID:	MGI:2655358
Gene symbol:	Il11ra1
Gene name:	interleukin 11 receptor subunit alpha 1

Probe:

Il11ra-pA, Il11ra-pB

Assay notes:

The purpose of this assay was to differentiate between Il11ra1 and Il11ra2 in the tissue by the differential pattern of restriction enzyme digestion. In this amplified fragment, Il11ra1 has a SacI site and Il11ra2 does not. The amplified products were undigested 170bp bands (-) or digested with Sac1 (+), Southern blotted and detected with a mixture of 2 probes. The probes were designed such that all restriction fragments would be hybridized.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	170 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
1-	Control
1+	P	TS28: bone marrow	Present	0.05 µg; total RNA	BALB/cBy		Not Specified
2-	Control
2+	P	TS28: bone marrow	Present	0.05 µg; total RNA	C3H/HeJ		Not Specified
3-	Control
3+	P	TS28: bone marrow	Present	0.05 µg; total RNA	C57BL/6J		Not Specified
4-	Control
4+	P	TS28: bone marrow	Present	0.05 µg; total RNA	129X1/SvJ		Not Specified
5-	Control
5+	P	TS28: bone marrow	Present	0.05 µg; total RNA	DBA/2J		Not Specified
6-	Control
6+	P	TS28: bone marrow	Present	0.05 µg; total RNA	SWR/J		Not Specified
7-	Control
7+	P	TS28: bone marrow	Present	0.05 µg; total RNA	SJL/J		Not Specified

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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