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Gene Expression Data
Assay Details
Assay
Reference: J:43018 Plummer NW, et al., Alternative splicing of the sodium channel SCN8A predicts a truncated two-domain protein in fetal brain and non-neuronal cells. J Biol Chem. 1997 Sep 19;272(38):24008-15
Assay type: RT-PCR
MGI Accession ID: MGI:2653618
Gene symbol: Scn8a
Gene name: sodium channel, voltage-gated, type VIII, alpha
Probe: Scn8a-pA, Scn8a-pB
Visualized with: Ethidium bromide
Assay notes: Transcript was detected in all tissues examined, but the splice forms detected varies.
Results
Image: 6 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 364 bp (a) 309 bp (b) 241 bp (c) Amount Genetic BackgroundMutant Allele(s)Sex
M Mol. Wt. Marker Lane
brain P adult TS28: brain Present Not Specified Not Specified 10 µg; total RNA Not Specified Not Specified
spinal cord P adult TS28: spinal cord Present Not Specified Not Specified 10 µg; total RNA Not Specified Not Specified
sciatic nerve P adult TS28: sciatic nerve Weak Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
liver P adult TS28: liver Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
kidney P adult TS28: metanephros Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
lung P adult TS28: lung Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
stomach P adult TS28: stomach Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
spleen P adult TS28: spleen Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
thymus P adult TS28: thymus Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Not Specified
testis P adult TS28: testis Not Specified Not Specified Not Specified 5 µg; total RNA Not Specified Male
water Control
Notes:
(a) This band contains exon 18A, the full length isoform. This is the predominant transcript in brain and spinal cord.
(b) This band contains exon 18N, which contains an in-frame stop codon and produces a truncated protein product. This is a predominant transcript in non-neural tissues.
(c) This band does not contain exon 18. The open reading frame is maintained, but the S3 and S4 segments of domain III are lacking. There are lower levels of this transcript than 18N in brain. This is a predominant transcript in non-neural tissues.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory