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Gene Expression Data
Assay Details
Assay
Reference: J:43018 Plummer NW, et al., Alternative splicing of the sodium channel SCN8A predicts a truncated two-domain protein in fetal brain and non-neuronal cells. J Biol Chem. 1997 Sep 19;272(38):24008-15
Assay type: RT-PCR
MGI Accession ID: MGI:2653616
Gene symbol: Scn8a
Gene name: sodium channel, voltage-gated, type VIII, alpha
Probe: Scn8a-pA, Scn8a-pB
Visualized with: Ethidium bromide
Results
Image: 2 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 364 bp (a) 309 bp (b) 241 bp (c) Amount Genetic BackgroundMutant Allele(s)Sex
M Mol. Wt. Marker Lane
E12.5 E12.5 (d) TS20: head Not Specified Present Present 10 µg; total RNA Not Specified Not Specified
E14.5 E14.5 (d) TS22: head Present Present Present 10 µg; total RNA Not Specified Not Specified
E18.5 E18.5 (d) TS26: brain Present Present Present 10 µg; total RNA Not Specified Not Specified
P1.5 P1.5 TS27: brain Present Present Present 10 µg; total RNA Not Specified Not Specified
P7.5 P7.5 TS28: brain Present Present Present 10 µg; total RNA Not Specified Not Specified
P20 P20 TS28: brain Present Present Present 10 µg; total RNA Not Specified Not Specified
adult P adult TS28: brain Present Present Present 10 µg; total RNA Not Specified Not Specified
water Control
Notes:
(a) This band contains exon 18A and produces the full-length transcript. This becomes the predominant transcript after E18.5.
(b) This band contains exon 18N, which contains and in-frame stop codon that generates a truncated protein. The level of this transcript decreases after birth.
(c) This band does not contain exon 18. It lacks the S3 and S4 segments of domain III. There are lower levels of this transcript than 18N in brain. The level of this transcript decreases after birth.
(d) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory