Map1b RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:65509 Gonzalez-Billault C, et al., Perinatal lethality of microtubule-associated protein 1B-deficient mice expressing alternative isoforms of the protein at low levels. Mol Cell Neurosci. 2000 Oct;16(4):408-21
Assay type:	RT-PCR
MGI Accession ID:	MGI:2652503
Gene symbol:	Map1b
Gene name:	microtubule-associated protein 1B

Probe:	3A-pA, 3A-pB
Probe preparation:	Antisense DNA
Visualized with:	Autoradiography
Assay notes:	After amplifying the cDNA using RT-PCR, the reaction products were blotted and then probed with a gene specific cDNA.

Results

Image: 3D 3A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	360 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E19.0 (a)	TS26: brain	Present	Not Specified; total RNA	Not Specified		Not Specified
+/-	E19.0 (a)	TS26: brain	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ * NMRI	Map1b^{Gt(IRESBetageo)2Pgr}/Map1b⁺	Not Specified
-/-	E19.0 (a)	TS26: brain	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ * NMRI	Map1b^{Gt(IRESBetageo)2Pgr}/Map1b^{Gt(IRESBetageo)2Pgr}	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels were slightly higher in the heterozygotic and homozygous mice compared to the wild-type. (In the wild-type animals the alternative transcripts are expressed at less than 10% of the level of the full length transcript.)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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