Map1b RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:65509 Gonzalez-Billault C, et al., Perinatal lethality of microtubule-associated protein 1B-deficient mice expressing alternative isoforms of the protein at low levels. Mol Cell Neurosci. 2000 Oct;16(4):408-21
Assay type:	RT-PCR
MGI Accession ID:	MGI:2652503
Gene symbol:	Map1b
Gene name:	microtubule-associated protein 1B

Probe:	3A-pA, 3A-pB
Probe preparation:	Antisense DNA
Visualized with:	Autoradiography
Assay notes:	After amplifying the cDNA using RT-PCR, the reaction products were blotted and then probed with a gene specific cDNA.

Results

Image: 3D 3A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	360 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E19.0 (a)	TS26: brain	Present	Not Specified; total RNA	Not Specified		Not Specified
+/-	E19.0 (a)	TS26: brain	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ * NMRI	Map1b^{Gt(IRESBetageo)2Pgr}/Map1b⁺	Not Specified
-/-	E19.0 (a)	TS26: brain	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ * NMRI	Map1b^{Gt(IRESBetageo)2Pgr}/Map1b^{Gt(IRESBetageo)2Pgr}	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels were slightly higher in the heterozygotic and homozygous mice compared to the wild-type. (In the wild-type animals the alternative transcripts are expressed at less than 10% of the level of the full length transcript.)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24