Map1b Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:65509 Gonzalez-Billault C, et al., Perinatal lethality of microtubule-associated protein 1B-deficient mice expressing alternative isoforms of the protein at low levels. Mol Cell Neurosci. 2000 Oct;16(4):408-21
Assay type:	Northern blot
MGI Accession ID:	MGI:2652496
Gene symbol:	Map1b
Gene name:	microtubule-associated protein 1B

Probe:	Map1B cDNA
Probe preparation:	Antisense labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 3B

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Size Not Specified (c)	Size Not Specified (d)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E19.0 (a)	TS26: brain	Present	Present	Present	15 µg; total RNA	Not Specified		Not Specified
+/-	E19.0 (a)	TS26: brain	Present	Present	Present	15 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * NMRI	Map1b^{Gt(IRESBetageo)2Pgr}/Map1b⁺	Not Specified
-/-	E19.0 (a)	TS26: brain	Trace	Absent	Absent	15 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * NMRI	Map1b^{Gt(IRESBetageo)2Pgr}/Map1b^{Gt(IRESBetageo)2Pgr}	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band corresponds to the full length transcript.
(c) This band corresponds to the 3U transcript.
(d) This band corresponds to the 3A transcript.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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