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Gene Expression Data
Assay Details
Assay
Reference: J:63702 Paulsen M, et al., Sequence conservation and variability of imprinting in the beckwith-wiedemann syndrome gene cluster in human and mouse. Hum Mol Genet. 2000 Jul 22;9(12):1829-41
Assay type: RT-PCR
MGI Accession ID: MGI:2451072
Gene symbol: Tspan32
Gene name: tetraspanin 32
Probe: Tssc6-pA, Tssc6-pB
Probe preparation: labelled with SYBR green
Assay notes: The gel was stained with Sybr Green I and scanned on a fluoroimager. Only 3 of the 5 alternative transcripts are amplified with these primers.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 756 bp (b) 756 bp (c) Amount Genetic BackgroundMutant Allele(s)Sex
BL6xSD7 Not Shown E16.5 (a) TS24: embryo Present Present Not Specified; total RNA (C57BL/6 x SD7)F1 Not Specified
BL6xSD7 Not Shown E16.5 (a) TS28: placenta Present Present Not Specified; total RNA (C57BL/6 x SD7)F1 Female
SD7xBL6 Not Shown E16.5 (a) TS24: embryo Present Present Not Specified; total RNA (SD7 x C57BL/6)F1 Not Specified
SD7xBL6 Not Shown E16.5 (a) TS28: placenta Present Present Not Specified; total RNA (SD7 x C57BL/6)F1 Female
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) B6 specific band.
(c) SD7 specific band; it cuts with TaiI
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory