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Gene Expression Data
Assay Details
Assay
Reference: J:63702 Paulsen M, et al., Sequence conservation and variability of imprinting in the beckwith-wiedemann syndrome gene cluster in human and mouse. Hum Mol Genet. 2000 Jul 22;9(12):1829-41
Assay type: RT-PCR
MGI Accession ID: MGI:2451042
Gene symbol: Tspan32
Gene name: tetraspanin 32
Probe: Tssc6-pA, Tssc6-pB
Probe preparation: labelled with SYBR green
Assay notes: The gel was stained with Sybr Green I and scanned on a fluoroimager. Only 3 of the 5 alternative transcripts are amplified with these primers.
Results Image: 6 Tssc6_Embryo d12.5
 Sample Information Bands Other Sample Information
Lane AgeStructure 756 bp (a) 756 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
BL6 Control
SD7 Control
BL6xSD7 Embryo E12.5 (c) TS20: embryo Present Present Not Specified; total RNA (C57BL/6 x SD7)F1 Not Specified
BL6xSD7 Placenta E12.5 (c) TS28: placenta Present Present Not Specified; total RNA (C57BL/6 x SD7)F1 Female
BL6xSD7 Embryo E12.5 (c) TS20: embryo Present Present Not Specified; total RNA (C57BL/6 x SD7)F1 Not Specified
BL6xSD7 Placenta E12.5 (c) TS28: placenta Present Present Not Specified; total RNA (C57BL/6 x SD7)F1 Female
SD7xBL6 Embryo E12.5 (c) TS20: embryo Present Present Not Specified; total RNA (SD7 x C57BL/6)F1 Not Specified
SD7xBL6 Placenta E12.5 (c) TS28: placenta Present Present Not Specified; total RNA (SD7 x C57BL/6)F1 Female
Notes:
(a) B6 specific band.
(b) SD7 specific band; it cuts with TaiI
(c) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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