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Gene Expression Data
Assay Details
Assay
Reference: J:80219 Voss AK, et al., The guanine nucleotide exchange factor C3G is necessary for the formation of focal adhesions and vascular maturation. Development. 2003 Jan;130(2):355-67
Assay type: RT-PCR
MGI Accession ID: MGI:2445473
Gene symbol: Rapgef1
Gene name: Rap guanine nucleotide exchange factor (GEF) 1
Probe: Grf2 pA, pB
Probe preparation: DNA
Visualized with: Ethidium bromide
Results
Image: 2B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 500 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
M Mol. Wt. Marker Lane
1 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129 * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1+Not Specified cDNA dilution 1:10
2 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129 * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1+Not Specified cDNA dilution 1:50
3 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129 * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1+Not Specified cDNA dilution 1:100
4 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129 * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1+Not Specified cDNA dilution 1:500; authors report prominent RT-PCR product
5 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129 * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1+Not Specified cDNA dilution 1:1000; authors report prominent RT-PCR product
6 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129/Sv * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5PgrNot Specified cDNA dilution 1:10; authors report small amount of RT-PCR product
7 E10.5 TS17: embryo Present Not Specified; total RNA involves: 129/Sv * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5PgrNot Specified cDNA dilution 1:50; authors report small amount of RT-PCR product
8 E10.5 TS17: embryo Absent Not Specified; total RNA involves: 129/Sv * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5PgrNot Specified cDNA dilution 1:100; authors report mRNA 3' of gene trap at < 1% of wild-type levels
9 E10.5 TS17: embryo Absent Not Specified; total RNA involves: 129/Sv * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5PgrNot Specified cDNA dilution 1:500
10 E10.5 TS17: embryo Absent Not Specified; total RNA involves: 129/Sv * C57BL/6 * Swiss Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5PgrNot Specified cDNA dilution 1:1000
11 Control
M Mol. Wt. Marker Lane
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory