Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Polktm1.1Rsky
Name:	polymerase (DNA directed), kappa; targeted mutation 1.1, Klaus Rajewsky
MGI ID:	MGI:2445458
Synonyms:	Polktm1.1Esp
Gene:	Polk  Location: Chr13:96617198-96679087 bp, - strand  Genetic Position: Chr13, 50.56 cM, cytoband D1
Alliance:	Polktm1.1Rsky page

Germline Transmission:	Earliest citation of germline transmission: J:80212
Parent Cell Line:	IB10/E14IB10 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: A loxP-flanked exon 6 and downstream floxed neo cassette were removed by crossing chimeric animals to a cre deleter strain resulting in the deletion of essential catalytic residues apartate 197 and glutamate 198, as well as a frameshift mutation via splicing from exon 5 to exon 7. RT-PCR analysis of testis RNA confirmed the presence of a shorter transcript in mutant animals. Northern blot analysis showed that the mutant transcript is expressed at 5 times less than the wild-type allele. (J:80212)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

16 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Polk Mutation:	65 strains or lines available

Original:	J:80212 Schenten D, et al., DNA polymerase kappa deficiency does not affect somatic hypermutation in mice. Eur J Immunol. 2002 Nov;32(11):3152-60
All:	4 reference(s)