Probe:	mouse class IV ADH cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent		2A	Authors report no expression during egg cylinder stage.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	2B	Expression first detected during gastrulation in posterior region, i.e. along primitive streak from anterior end (node), posteriorly to allantois.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2C
TS11: extraembryonic component	Absent		2C

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	2D	Expression posteriorly in primitive streak; posterior boundary marked by allantois.
TS12: future hindbrain neural fold	Present	Regionally restricted	2D	Authors report expression anteriorly in neural folds,
TS12: future midbrain neural fold	Present	Regionally restricted	2D	Authors report expression anteriorly in neural folds,
TS12: future prosencephalon neural fold	Present	Regionally restricted	2D	Authors report expression anteriorly in neural folds,

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural fold	Present		2E
TS12: future midbrain neural fold	Present		2E
TS12: future prosencephalon neural fold	Present		2E

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	2F	Expression in dorsolateral regions of neural folds and craniofacial region, as well as in trunk and caudal neuropore.
TS13: neural ectoderm	Present	Regionally restricted	2F	Expression in dorsolateral regions of neural folds and craniofacial region, as well as in trunk and caudal neuropore.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural fold	Present	Regionally restricted	2G	Expression in dorsolateral regions of neural folds.
TS14: embryo	Present	Regionally restricted	2G	Authors report expression in craniofacial region, trunk, and caudal neuropore.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	2H	Authors report expression in posterior trunk.
TS15: forelimb bud	Present		2H
TS15: head mesenchyme	Present	Regionally restricted	2H	Expression in the head limited to ventral craniofacial structures (eg. frontonasal mass, perioptic region).
TS15: 1st branchial arch mandibular component	Present		2H
TS15: 1st branchial arch maxillary component	Present		2H
TS15: future brain	Absent		2H

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	3A	Expression localized primarily to primitive streak mesoderm extending from approximately the center of the embryo (node) to the allantois.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	3B	In the neuroepithelium of future forebrain and hindbrain, expression is along the dorsolateral edge nearest to cranial mesenchyme corresponding to site of cranial neural crest migration.
TS12: future prosencephalon	Present	Regionally restricted	3B	In the neuroepithelium of future forebrain and hindbrain, expression is along the dorsolateral edge nearest to cranial mesenchyme corresponding to site of cranial neural crest migration.
TS12: future hindbrain neural crest	Present		3B
TS12: future midbrain neural crest	Present		3B
TS12: future prosencephalon neural crest	Present		3B
TS12: head mesenchyme derived from neural crest	Present		3B
TS12: mesenchyme derived from head mesoderm	Absent		3B	No expression in the midline mesoderm underlying the neural groove.
TS12: foregut diverticulum endoderm	Absent		3B

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3C	Expression in cranial neural folds of forebrain and hindbrain.
TS13: future prosencephalon	Present	Regionally restricted	3C	Expression in cranial neural folds of forebrain and hindbrain.
TS13: head mesenchyme derived from neural crest	Present	Regionally restricted	3C	Expression localized primarily to perioptic neural crest mesenchyme surrounding optic pit.
TS13: 1st branchial arch	Present		3C
TS13: 2nd branchial arch	Present		3C

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	3D	Expression in cranial mesenchyme; greater expression in mesenchyme of frontonasal, perioptic and branchial arch regions.
TS15: 1st branchial arch mandibular component	Present		3D
TS15: 1st branchial arch maxillary component	Present		3D
TS15: 2nd branchial arch	Present		3D
TS15: future brain	Absent		3D	Close to background expression in neuroepithelium of all regions of brain and optic vesicle.
TS15: optic vesicle	Absent		3D	Close to background expression in neuroepithelium of all regions of brain and optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	3E	Expression in trunk primarily localized to paraxial mesoderm, particularly in the most posterior portion of trunk where somites had not yet formed, as well as in somites posterior to forelimb bud.
TS15: trunk somite	Present	Regionally restricted	3E	Expression in trunk primarily localized to paraxial mesoderm, particularly in the most posterior portion of trunk where somites had not yet formed, as well as in somites posterior to forelimb bud.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	3F	Expression in forelimb bud localized primarily to proximal rather than distal mesenchyme; no anterior-posterior difference was noted.