Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Thratm2Jas
Name:	thyroid hormone receptor alpha; targeted mutation 2, Jacques Samarut
MGI ID:	MGI:2183135
Synonyms:	TRalpha0
Gene:	Thra  Location: Chr11:98631539-98659832 bp, + strand  Genetic Position: Chr11, 62.58 cM, cytoband D-E
Alliance:	Thratm2Jas page

Germline Transmission:	Earliest citation of germline transmission: J:70007
Parent Cell Line:	ENS (ES Cell)
Strain of Origin:	129/Sv

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: Exons 5 through 7 were replaced with a cassette comprised of lacZ and neo. The targeting vector was designed to abolish transcription of the four known isoforms produced from this locus. The deleted region encoded the DNA-binding domain found in the two larger isoforms and the internal promoter in intron 7 that drives the transcription of the two truncated isoforms. Northern blot and RT-PCR analyses of intestinal RNA showed the absence of all four transcripts in homozygous mutant mice. RNA protection assays revealed the occasional presence of the truncated transcripts resulting from sporadic readthrough of the stop codons contained within the inserted cassette. (J:70007)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	18 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	17 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Thra Mutation:	39 strains or lines available

Original:	J:70007 Gauthier K, et al., Genetic analysis reveals different functions for the products of the thyroid hormone receptor alpha locus. Mol Cell Biol. 2001 Jul;21(14):4748-60
All:	32 reference(s)