Gene Expression Data
Assay Details
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Assay
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Reference:
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J:77124
Shinozaki K, et al., Absence of Cajal-Retzius cells and subplate neurons associated with defects of tangential cell migration from ganglionic eminence in Emx1/2 double mutant cerebral cortex. Development. 2002 Jul;129(14):3479-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2182356
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Gene symbol:
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Map2
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Gene name:
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microtubule-associated protein 2
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Results
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Specimen
11G:
embryonic day 12.5
(more  )
Specimen
11G:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebral cortex |
Present |
Regionally restricted |
11G
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Expression is in the preplate pioneer neurons.
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Specimen
11H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebral cortex |
Present |
Regionally restricted |
11H
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Expression is in the preplate pioneer neurons. Expression in this mutant is the same as in wild-type embryos.
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Specimen
11I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx1tm1Sia/Emx1tm1Sia
Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebral cortex |
Present |
Regionally restricted |
11I
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Expression is on the outer surface of the neuroepithelium, similar to wild-type embryos.
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Specimen
7I:
embryonic day 18.5
(more )
Specimen
7I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7J:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx1tm1Sia/Emx1tm1Sia
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex marginal zone |
Absent |
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7J
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Staining in this mutant is the same as in wild-type embryos.
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| TS26: cortical plate |
Present |
Regionally restricted |
7J
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Staining is in neurons that are arranged radially and form a tight, pallisade-like pattern in the cortical plate.
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| TS26: cerebral cortex |
Present |
Regionally restricted |
7J
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Staining is also in the horizontal processes of subplate neurons. Staining in this mutant is the same as in wild-type embryos.
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Specimen
7K:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7L:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx1tm1Sia/Emx1tm1Sia
Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex ventricular zone |
Absent |
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7L
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| TS26: cerebral cortex |
Present |
Regionally restricted |
7L
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In this mutant the pattern of expression is severely disordered. Expression is scattered throughout the neocortical wall. The palisade-like pattern and the subplate neurons have disappeared. There is strong expression just beneath the pial surface.
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| TS26: cerebral cortex marginal zone |
Present |
Regionally restricted |
7L
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In this mutant the pattern of expression is severely disordered. Expression is scattered throughout the neocortical wall. The palisade-like pattern and the subplate neurons have disappeared. There is strong expression just beneath the pial surface.
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Analysis Tools