Summary | Mutation origin | Mutation description | Expression | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Trp53tm1Ldo
Name:	transformation related protein 53; targeted mutation 1, Lawrence A Donehower
MGI ID:	MGI:2159430
Synonyms:	p53m
Gene:	Trp53  Location: Chr11:69471185-69482699 bp, + strand  Genetic Position: Chr11, 42.83 cM, cytoband B2-C
Alliance:	Trp53tm1Ldo page

Germline Transmission:	Earliest citation of germline transmission: J:73757
Parent Cell Line:	AB1 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1+

Allele Type:    Targeted Mutations:    Insertion, Intragenic deletion   Mutation details: Insertion of a PGK-hypoxanthine phosphoribosyltransferase (HPRT) expression cassette introduced into exon 7 an A-to-T mutation that results in a p.Arg245Trp substitution, and deleted 20 kb of sequence that included exons 1-6 and a large undefined upstream region. The authors propose that an unknown upstream promoter initiates a chimeric mRNA containing a 55-nucleotide leader spliced onto exons 7-11. This mRNA contains three in-frame AUG codons. The third AUG codon is a viable translation initiation site, which may produce a C-terminal protein fragment. RT-PCR analyses detect expression of this allele in several tissues of heterozygous mutant mice, albeit at lower levels than the wild-type allele. Studies of in vitro transcription and translation of a construct containing cDNA from this allele detected expression of a predicted 24 kDa protein. (J:73757)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

12 anatomical structure(s)

List all tumor models in MMHCdb carrying Trp53tm1Ldo

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Trp53 Mutation:	232 strains or lines available

Original:	J:73757 Tyner SD, et al., p53 mutant mice that display early ageing-associated phenotypes. Nature. 2002 Jan 3;415(6867):45-53
All:	11 reference(s)