Probe:	exon 2 probe A
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Authors report that all results were confirmed using exon2 probe C (MGI:2153549).

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS21: brain	Present		1A	Authors report expression throughout the brain.
TS21: cerebral cortex	Present		1A
TS21: midbrain mantle layer	Present		1A
TS21: midbrain ventricular layer	Present		1A
TS21: hindbrain	Present	Regionally restricted	1A	Expression in mantle and ventricular zones.
TS21: spinal cord mantle layer	Present		1A
TS21: optic nerve	Present		1A
TS21: cervical ganglion	Present		1A
TS21: peripheral nerve trunk	Present		1A
TS21: spinal nerve	Present		1A
TS21: gut	Present	Regionally restricted	1A	Expression in submucosal layers of the intestine.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present	Regionally restricted		Associated with nervous layer containing large number of sensory neurons.
TS21: tongue	Present	Regionally restricted		Associated with submucosal nerve plexus on dorsum of tongue.
TS21: upper jaw tooth	Weak			Diffuse low level. Detected in early stages of differentiation of dental lamina.
TS21: lower jaw tooth	Present	Regionally restricted		Detected in early stages of differentiation of dental lamina.

Structure	Level	Pattern	Image	Note
TS24: superior cervical ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present	Regionally restricted	2C	Associated with large neuronal cells.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		2E
TS24: pituitary gland	Present		2E
TS24: trigeminal ganglion	Present		2E
TS24: trigeminal nerve	Present		2E

Structure	Level	Pattern	Image	Note
TS24: optic nerve	Present		2G

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	3A	Localized to region containing large numbers of neurons.

Structure	Level	Pattern	Image	Note
TS24: tongue	Present	Regionally restricted	3C	Hybridization in the submucous nerve plexus on dorsum of tongue.

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Present	Regionally restricted	3E	Associated with the inner nuclear layer.
TS24: pigmented retinal epithelium	Absent		3E

Structure	Level	Pattern	Image	Note
TS24: lower jaw tooth	Present	Regionally restricted	4A	Hybridization in inner and outer epithelium layers of tooth primordium, which give rise to enamel-forming cells.
TS24: upper jaw tooth	Present	Regionally restricted	4A	Hybridization in inner and outer epithelium layers of tooth primordium, which give rise to enamel-forming cells.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present	Regionally restricted	4C	Authors report hybridization in metanephric cap derivatives.

Structure	Level	Pattern	Image	Note
TS24: brain	Present			Authors report transcripts throughout the brain.
TS24: central nervous system nerve	Present	Regionally restricted		Transcripts in nerve axons of central and peripheral nervous systems.
TS24: peripheral nervous system	Present	Regionally restricted		Transcripts in nerve axons of central and peripheral nervous systems.
TS24: gut	Present	Regionally restricted		Transcripts in the intestine, associated with sympathetic plexus and parasympathetic ganglia.
TS24: parasympathetic nervous system	Present	Regionally restricted		Transcripts in the intestine, associated with sympathetic plexus and parasympathetic ganglia.
TS24: sympathetic nervous system	Present	Regionally restricted		Transcripts in the intestine, associated with sympathetic plexus and parasympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present			Transcripts detected in maternal decidual cells surrounding the embryos and their membranes.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present			Transcripts detected in maternal decidual cells surrounding the embryos and their membranes.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present			Transcripts detected in the maternal decidua.
TS21: extraembryonic component	Present	Regionally restricted		Also detected in other extra-embryonic tissues: amnion, umbilical cord and mesodermal layer of yolk sac.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		4E	Transcripts detected in the maternal decidua.

Structure	Level	Pattern	Image	Note
TS24: extraembryonic component	Present	Regionally restricted	4G	Transcripts detected in the amnion, mesodermal layer of the yolk sac and the umbilical cord.