Gene Expression Data
Assay Details
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Assay
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Reference:
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J:71034
Boyl PP, et al., Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR. Development. 2001 Aug;128(15):2989-3000
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2150149
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Antibody:
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anti-OTX2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3A' wt:
embryonic day 6.5
(more  )
Specimen
3A' wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3B' Otx2 +/-:
embryonic day 6.5; Otx2tm1Pas/Otx2 +
(more )
Specimen
3B' Otx2 +/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2+
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3C' Otx2 l/l:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm2Asim/Otx2tm2Asim
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3D' Otx2 l/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm2Asim
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3E',I wt:
embryonic day 7.75
(more )
Specimen
3E',I wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3F',J Otx2 +/-:
embryonic day 7.75; Otx2tm1Pas/Otx2 +
(more )
Specimen
3F',J Otx2 +/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2+
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Age:
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embryonic day 7.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3G',K Otx2 l/l:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm2Asim/Otx2tm2Asim
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Age:
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embryonic day 7.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
3H',L Otx2 l/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm2Asim
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Age:
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embryonic day 7.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
3L
,
3H'
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Expression is only seen in the rostral-most neuroectoderm. It displays a heterogenity in the strength of staining, and expression levels are severely reduced compared to those seen in wild type.
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| TS11: mesendoderm |
Present |
Regionally restricted |
3H'
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3L
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Expression is anterior; expression levels are severely reduced compared to those seen in wild type.
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| TS11: extraembryonic visceral endoderm |
Present |
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3H'
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Expression levels are comparable to those seen in wild type.
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