Ebf1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type:	RNA in situ
MGI Accession ID:	MGI:2148276
Gene symbol:	Ebf1
Gene name:	early B cell factor 1

Probe:	Ebf cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

2I ©

Specimen 2I: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2I	Expression in anterior pretectum, anterior tectum and tegmentum.

2J ©

Specimen 2J: embryonic day 11.5; Emx2^tm1Sia/Emx2^tm1Sia, Otx2^tm1Sia/Otx2⁺ (more

)
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2J	Expression in anterior tectum and tegmentum; absent in anterior pretectum.

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