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Gene Expression Data
Assay Details
Assay
Reference: J:69854 Liu C, et al., Regulation of left-right asymmetry by thresholds of Pitx2c activity. Development. 2001 Jun;128(11):2039-48
Assay type: RNase protection
MGI Accession ID: MGI:2137364
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Probe: Pitx2c
Probe preparation: Antisense DNA
Assay notes: A probe that specifically recognizes the c isoform was used. Fig 1Q is a quantitation of the mRNA bands in Fig 1P.
Results
Image: 1P ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
1 Control
2 Control
3 Control
4 E12.5 (a) TS20: embryo Present Not Specified Not Specified Not Specified
5 E12.5 (a) TS20: embryo Present Not Specified Not Specified Not Specified
6 E12.5 (a) TS20: embryo Present (b) Not Specified involves: 129S4/SvJaeSor Pitx2tm1Jfm/Pitx2+Not Specified
7 E12.5 (a) TS20: embryo Moderate (c) Not Specified involves: 129S4/SvJaeSor * C57BL/6J Pitx2tm1Jfm/Pitx2tm2.1JfmNot Specified
8 E12.5 (a) TS20: embryo Moderate (c) Not Specified involves: 129S4/SvJaeSor * C57BL/6J Pitx2tm1Jfm/Pitx2tm2.1JfmNot Specified
9 E12.5 (a) TS20: embryo Moderate (d) Not Specified involves: 129S4/SvJaeSor * C57BL/6J Pitx2tm1Jfm/Pitx2tm2JfmNot Specified
10 E12.5 (a) TS20: embryo Moderate (d) Not Specified involves: 129S4/SvJaeSor * C57BL/6J Pitx2tm1Jfm/Pitx2tm2JfmNot Specified
11 E12.5 (a) TS20: embryo Absent Not Specified involves: 129S4/SvJaeSor Pitx2tm1Jfm/Pitx2tm1JfmNot Specified
12 E12.5 (a) TS20: embryo Absent Not Specified involves: 129S4/SvJaeSor Pitx2tm1Jfm/Pitx2tm1JfmNot Specified
13 Mol. Wt. Marker Lane
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) These had 58% less mRNA than the wild type ones in lanes 4,5.
(c) These had 50% less mRNA than the heterozygotes in lanes 6.
(d) These had 37% less mRNA than the heterozygotes in lanes 6.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory