Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	MipGt(OST1231)Lex
Name:	major intrinsic protein of lens fiber; gene trap OST1231, Lexicon Genetics
MGI ID:	MGI:1861978
Synonyms:	AQP0-, MipGt(Betageo)513Lex, MipGt(VICTR20)8Lex, Miptm1Lex
Gene:	Mip  Location: Chr10:128061707-128067681 bp, + strand  Genetic Position: Chr10, 76.49 cM, cytoband D1
Alliance:	MipGt(OST1231)Lex page

Mutant Cell Line:	OST1231
Germline Transmission:	Earliest citation of germline transmission: J:84775
Parent Cell Line:	Lex-1 (ES Cell)
Strain of Origin:	129S5/SvEvBrd
Project Collection:	Lexicon

Allele Type:    Gene trapped (Null/knockout, Reporter) Mutation:    Insertion of gene trap vector   Mutation details: A gene trap vector consisting of LTR sites flanking a beta-geo cassette containing a splice acceptor and a PGK-puro cassette containing a splice donor inserted into exon 1 of the endogenous gene, 168 nucleotides downstream of the start codon. The integration generated a nonsense mutation at codon 57, precluding the translation of ~79% (207 residues) of the protein, including the functionally conserved asparagine-proline-alanine motifs located at codons 68-70 and 184-186. Northern and Western blot analyses indicated an absence of normal transcript and protein in homozygous mutant mice. (J:84775) Sequence Tags:  Sequence tag details (1 tag) Tag ID GenBank ID Method Tag Location (trapped strand)* Select OST1231 CG472657 (MGI Seq Detail) 3' RACE Chr10:128062916-128066374 bp (+) * GRCm39 (strand of gene trap mutagenesis) Selected Tags: download BLAST at NCBI Genome Context:  Genome Browser view of this mutation 24 gene trap insertions have trapped this gene View all gene trap sequence tags in this region

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Mip Mutation:	31 strains or lines available

Original:	J:84775 Shiels A, et al., Optical dysfunction of the crystalline lens in aquaporin-0-deficient mice. Physiol Genomics. 2001 Dec 21;7(2):179-86
All:	14 reference(s)