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Gene Expression Data
Assay Details
Assay
Reference: J:53337 Yuan SS, et al., Cloning and functional studies of a novel gene aberrantly expressed in RB-deficient embryos. Dev Biol. 1999 Mar 1;207(1):62-75
Assay type: RNA in situ
MGI Accession ID: MGI:1343366
Gene symbol: Robo3
Gene name: roundabout guidance receptor 3
Probe: Rig-1 oligo
Probe preparation: Antisense labelled with P33 RNA
Visualized with: Autoradiography
Assay notes: The probe used in this assay was 3' labelled.
Results
Specimen 2A - 2C: embryonic day 11.5; Rb1tm1Brd/Rb1tm1Brd (more )
Note: Fig. 2A - brightfield, 2C - darkfield, 2B - superimposition of brightfield and darkfield.
Structure Level Pattern Image Note
TS19: heart Absent 2B , 2C , 2A
TS19: liver Absent 2B , 2C , 2A
TS19: lung Absent 2B , 2C , 2A

Specimen 2D - 2L: embryonic day 11.5; Rb1tm1Brd/Rb1tm1Brd (more )
Note: Fig. 2D,G,J - brightfield, 2F,I,L - darkfield, 2E,H,K - superimposition of brightfield and darkfield. Fig. G-I and J-L are higher magnification images of the boxed regions in Fig. D.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 2G , 2H , 2D , 2I , 2J , 2E , 2L , 2F , 2K Rig-1 transcripts were expressed in the region between the proliferative cell-containing ventricular zone and the postmitotic cell-containing migration zone.
TS19: hindbrain Present Regionally restricted 2G , 2H , 2D , 2I , 2J , 2E , 2L , 2F , 2K Rig-1 transcripts were expressed in the region between the proliferative cell-containing ventricular zone and the postmitotic cell-containing migration zone.
TS19: heart Absent 2E , 2D , 2F
TS19: limb Absent 2E , 2D , 2F

Specimen 2M - 2O: embryonic day 11.5 (more )
Note: Fig. 2M - brightfield, 2O - darkfield, 2N - superimposition of brightfield and darkfield.
Structure Level Pattern Image Note
TS19: embryo Present 2M , 2N , 2O

Specimen 2P - 2R: embryonic day 11.5; Rb1tm1Brd/Rb1tm1Brd (more )
Note: Fig. 2P - brightfield, 2R - darkfield, 2Q - superimposition of brightfield and darkfield.
Structure Level Pattern Image Note
TS19: embryo Present 2Q , 2R , 2P Expression in this mutant is similar to that in wild-type embryos.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory