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Gene Expression Data
Assay Details
Assay
Reference: J:26101 Hacker A, et al., Expression of Sry, the mouse sex determining gene. Development. 1995 Jun;121(6):1603-14
Assay type: RNase protection
MGI Accession ID: MGI:1335066
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Probe: Sry probe2
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Quantification of these results is shown in Figure 5B. 4 pairs of genital ridges were used to make RNA samples.
Results
Image: 5A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 270 bp 97 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
B E3.5 TS4: blastocyst-stage conceptus Absent Absent Not Specified; total RNA Parkes Not Specified 225 blastocysts were used in this lane.
5 E10.5 (a) TS17: gonad primordium Absent Absent Not Specified; total RNA Parkes Male
8 E10.5 (b) TS17: gonad primordium Absent Absent Not Specified; total RNA Parkes Male
11/12 E10.5 (c) TS17: gonad primordium Weak Absent Not Specified; total RNA Parkes Male
14 E11.0 (d) TS18: gonad primordium Present Absent Not Specified; total RNA Parkes Male
16 E11.5 (e) TS19: gonad Present Absent Not Specified; total RNA Parkes Male
18 E11.5 (f) TS19: gonad Strong Absent Not Specified; total RNA Parkes Male
21 E11.5 (g) TS19: gonad Present Absent Not Specified; total RNA Parkes Male
24 E12.0 (h) TS20: testis Present Absent Not Specified; total RNA Parkes Male
27 E12.5 (i) TS20: testis Weak Absent Not Specified; total RNA Parkes Male
32 E12.5 (j) TS20: testis Absent Absent Not Specified; total RNA Parkes Male
7pp P7 TS28: testis Absent Absent 5 µg; total RNA Parkes Male
14pp P14 TS28: testis Absent Absent 5 µg; total RNA Parkes Male
21pp P21 TS28: testis Absent Absent 5 µg; total RNA Parkes Male
28pp P28 TS28: testis Absent Present 5 µg; total RNA Parkes Male
43pp P43 TS28: testis Absent Present 5 µg; total RNA Parkes Male
L P adult TS28: liver Absent Absent 5 µg; total RNA Parkes Male
Notes:
(a) 5 somites
(b) 8 somites
(c) 11/12 somites
(d) 14 somites
(e) 16 somites
(f) 18 somites
(g) 21 somites
(h) 24 somites
(i) 27 somites
(j) 32 somites
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory