About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:26101 Hacker A, et al., Expression of Sry, the mouse sex determining gene. Development. 1995 Jun;121(6):1603-14
Assay type: RNase protection
MGI Accession ID: MGI:1335066
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Probe: Sry probe2
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Quantification of these results is shown in Figure 5B. 4 pairs of genital ridges were used to make RNA samples.
Results
Image: 5A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 270 bp 97 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
B E3.5 TS4: blastocyst-stage conceptus Absent Absent Not Specified; total RNA Parkes Not Specified 225 blastocysts were used in this lane.
5 E10.5 (a) TS17: gonad primordium Absent Absent Not Specified; total RNA Parkes Male
8 E10.5 (b) TS17: gonad primordium Absent Absent Not Specified; total RNA Parkes Male
11/12 E10.5 (c) TS17: gonad primordium Weak Absent Not Specified; total RNA Parkes Male
14 E11.0 (d) TS18: gonad primordium Present Absent Not Specified; total RNA Parkes Male
16 E11.5 (e) TS19: gonad Present Absent Not Specified; total RNA Parkes Male
18 E11.5 (f) TS19: gonad Strong Absent Not Specified; total RNA Parkes Male
21 E11.5 (g) TS19: gonad Present Absent Not Specified; total RNA Parkes Male
24 E12.0 (h) TS20: testis Present Absent Not Specified; total RNA Parkes Male
27 E12.5 (i) TS20: testis Weak Absent Not Specified; total RNA Parkes Male
32 E12.5 (j) TS20: testis Absent Absent Not Specified; total RNA Parkes Male
7pp P7 TS28: testis Absent Absent 5 µg; total RNA Parkes Male
14pp P14 TS28: testis Absent Absent 5 µg; total RNA Parkes Male
21pp P21 TS28: testis Absent Absent 5 µg; total RNA Parkes Male
28pp P28 TS28: testis Absent Present 5 µg; total RNA Parkes Male
43pp P43 TS28: testis Absent Present 5 µg; total RNA Parkes Male
L P adult TS28: liver Absent Absent 5 µg; total RNA Parkes Male
Notes:
(a) 5 somites
(b) 8 somites
(c) 11/12 somites
(d) 14 somites
(e) 16 somites
(f) 18 somites
(g) 21 somites
(h) 24 somites
(i) 27 somites
(j) 32 somites
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory