Sry RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:26101 Hacker A, et al., Expression of Sry, the mouse sex determining gene. Development. 1995 Jun;121(6):1603-14
Assay type:	RNase protection
MGI Accession ID:	MGI:1335065
Gene symbol:	Sry
Gene name:	sex determining region of Chr Y

Probe:	Sry probe2
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography
Assay notes:	This probe highlights the multiple transcription start sites of the genital ridge transcript and the splice sites of the testis transcript.

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	371 bp (a)	270 bp (b)	97 bp (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Probe 2 L	P adult	TS28: liver	Absent	Absent	Absent	5 µg; total RNA	Parkes		Not Specified
Probe 2 G	E11.5	TS19: gonad	Weak	Present	Absent	Not Specified; total RNA	Parkes		Pooled	5 pairs of ridges
Probe 2 T	P adult	TS28: testis	Weak	Absent	Present	5 µg; total RNA	Parkes		Male

Notes:
(a) This band may be due to a far upstream transcription start site.
(b) This cluster of bands may correspond to multiple transcription start sites.
(c) This band is due to an alternate splice site at position bp 8201.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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