Gene Expression Data
Assay Details
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Assay
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Reference:
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J:18196
Wallin J, et al., The role of Pax-1 in axial skeleton development. Development. 1994 May;120(5):1109-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1334713
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Antibody:
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Anti Pax1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The primary antibody was diluted 1:200 in blocking solution. Embryos were fixed in 3% acetic acid in absolute ethanol.
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Results
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Specimen
Not Shown 9.5dpc:
embryonic day 9.5
(more  )
Specimen
Not Shown 9.5dpc:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: sclerotome |
Present |
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1B
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Expression is in almost all sclerotome cells.
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tail sclerotome |
Present |
Regionally restricted |
1C
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Expression is in the cranial half of each lateral sclerotome.
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Specimen
Not Shown new-born:
postnatal newborn
(more )
Specimen
Not Shown new-born:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: skeletal system |
Present |
Regionally restricted |
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These data are described in the text. Expression is in the annulus fibrosus of the vertebral column.
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Specimen
7E:
embryonic day 12.5; Pax1un/Pax1 +
(more )
Specimen
7E:
(close )
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Genetic Background:
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involves: C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Pax1un/Pax1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
7E
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Expression is strong in the anlagen of the intervertebral discs. There is no expression in the anlagen of the vertebral bodies.
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Specimen
7F:
(close )
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Genetic Background:
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involves: C3HeB/FeJ * C57BL/6 * CC57BR/MvY
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Mutant Allele(s):
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Pax1Un-s/Pax1un
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
7F
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Expression domains in the mutant are much wider in the craniocaudal dimension.
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Specimen
Not Shown 12.5dpc:
(close )
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Genetic Background:
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involves: C57BL/6 * CC57BR/MvY
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Mutant Allele(s):
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Pax1Un-s/Pax1Un-s
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
7G:
embryonic day 14.5; Pax1un/Pax1 +
(more )
Specimen
7G:
(close )
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Genetic Background:
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involves: C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Pax1un/Pax1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tail sclerotome |
Present |
Regionally restricted |
7G
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Expression is in the cranial half of each lateral sclerotome.
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Specimen
7H:
(close )
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Genetic Background:
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involves: C3HeB/FeJ * C57BL/6 * CC57BR/MvY
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Mutant Allele(s):
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Pax1Un-s/Pax1un
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tail sclerotome |
Present |
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7H
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In mutant embryos, the lateral lumbosacral sclerotome domains are altered and dense layers of cells expressing Pax1 are found surrounding the spinal nerve.
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