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Gene Expression Data
Assay Details
Assay
Reference: J:41159 Opdecamp K, et al., Melanocyte development in vivo and in neural crest cell cultures: crucial dependence on the Mitf basic-helix-loop-helix-zipper transcription factor. Development. 1997 Jun;124(12):2377-86
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1332787
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-CD117 (ACK2)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Fresh frozen sections were fixed with methanol (100%, 15 minutes, -20C) followed by 2% paraformaldehyde (2 minutes, room temperature).
Results
Specimen 6.A.: embryonic day 10.5 (more )
Note: Double labeled: green - Kit; red - Mitf (assay MGI:1332789).
Structure Level Pattern Image Note
TS17: trunk mesenchyme Present Spotted 6A Both Kit single-positive and Kit/Mitf double-positive cells are detected.
Specimen 6.B.: embryonic day 10.5; Mitfmi-di/Mitfmi-di (more )
Note: Double labeled: green - Kit; red - Mitf (assay MGI:1332789).
Structure Level Pattern Image Note
TS17: trunk mesenchyme Present Spotted 6B Fewer Kit positive cells are found than in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory